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Survey: Sperm DNA Fragmentation
Assessment of Sperm DNA Fragmentation by TUNEL, SCSA, halosperm or other appear very popular but is it? Complete the survey to find out just how many clinics are performing this assay. This survey may be presented at the 10th International Congress of Andrology. 23 26 February 2013 Melbourne, Australia. Please use the comments section to describe how you do sperm DNA Fragmentation assessment and what are your cut-offs.
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The Question was:In-house or Outsourcing tests - Total yes replies= 23
1. The Option: Yes I perform some form of Sperm DNA Assessment in my laboratory or clinic. [Results: Yes= 13 No: 10 Total= 23] ANALYSIS
2. The Option: No, we do NOT perform a sperm DNA Fragmentation Assay in our lab but outsource it to another lab. [Results: Yes= 6 No: 17 Total= 23] ANALYSIS
3. The Option: NO we do not use sperm DNA Fragmentation assay in our clinical management!! [Results: Yes= 4 No: 19 Total= 23] ANALYSIS
The Question was:Type of DNA Testing - Total yes replies= 19
4. The Option: We use TUNEL methodology [Results: Yes= 3 No: 16 Total= 19] ANALYSIS
5. The Option: We use SCSA (Sperm chromatin structure assay) in our lab. [Results: Yes= 4 No: 15 Total= 19] ANALYSIS
6. The Option: We use Halosperm in our laboratory. [Results: Yes= 9 No: 10 Total= 19] ANALYSIS
7. The Option: We use a Comet methodology [Results: Yes= 1 No: 18 Total= 19] ANALYSIS
8. The Option: We use a different method to those listed above (describe the method in the comments section below) [Results: Yes= 2 No: 17 Total= 19] ANALYSIS
The Question was:Application. - Total yes replies= 20
9. The Option: We perform it as a screening test for all new clients [Results: Yes= 5 No: 15 Total= 20] ANALYSIS
10. The Option: We perform it prior to an IVF cycle [Results: Yes= 2 No: 18 Total= 20] ANALYSIS
11. The Option: We perform it only after a poor IVF outcome [Results: Yes= 3 No: 17 Total= 20] ANALYSIS
12. The Option: We perform it only when order by a doctor. [Results: Yes= 8 No: 12 Total= 20] ANALYSIS
13. The Option: We perform it before all new IUI cycles. [Results: Yes= 2 No: 18 Total= 20] ANALYSIS
The Question was:Donor Testing. - Total yes replies= 15
14. The Option: We assess all new sperm donors for sperm DNA Fragmentation. [Results: Yes= 7 No: 8 Total= 15] ANALYSIS
15. The Option: We do not assess all new sperm donors. [Results: Yes= 8 No: 7 Total= 15] ANALYSIS
#28 of Germany writes: we encourage all new clients and those with a poor outcome (poor embryo development, biochemical pregnancy, early abortion) to take the test, but patients have to pay for it out out of their own pocket so many prefer not to go ahead.
#29 of Australia writes: We have instituted SCSA as a screening test for all new semen analysis as part of a clinic wide protocol. If abnormal, the men usually undergo vitamin treatment but unfortunately are not retested. Abnormal SCSA >15% always go to ICSI
#100 of India writes: initially in 2009- 2010 i did halosperm test for couple with poor reproductive outcome both in spontaneous and ART pregnancies and found DFI <20% had better prognosis [ among indian clients]. Now i don"t do halosperm test, instead i put the husband on anti oxidants and advise life style modification.
#101 of Peru writes: The test used by us is SCD
#1142 writes: Very interesting and useful discussion!
#1143 of India writes: which one is the best protocol for achieving fair success rate in IUI cycles
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