List of Recent Publications. There are 20 links to this search
New Frontiers in IVF: mtDNA and autologous germline mitochondrial energy transfer 
Ovarian aging leads to a decrease in the quantity and quality of oocytes and aged oocytes have a reduced number of mitochondria. Mitochondria are the energy factories of the cells and their lacked could leads to lower fertilization rates and poor embryonic development. Various strategies have been tested to increase the mitochondria quantity and thus improve the quality of oocytes used in in vitro fertilization. Results of ovarian rejuvenation te
Factors influencing dynamics of follicular pool development 
Published in Frontiers in Endocrinology (2019).Growth Hormone and Reproduction: Lessons Learned From Animal Models and Clinical Trials. Dosouto C, Calaf J, Polo A, Haahr T, Humaidan P. Front Endocrinol (Lausanne). 2019 Jun 26:10:404. doi: 10.3389/fendo.2019.00404.
Growth Hormone and Reproduction: Lessons Learned From Animal Models and Clinical Trials 
Evidence suggests that GH can stimulate the secretion of IGF1 not only in the liver but also in the peripheral target structures, including the ovary. Moreover, IGF-1 can be secreted locally under the influence of stimuli other than GH. In the case of the ovary, steroid hormones, gonadotropins or the combination of both seems to be involved. Even more interesting, the ovary itself can secret GH locally and exert a paracrine action modulating the
Association between the number of retrieved mature donor oocytes and live birth in IVF donor recipient cycles using frozen donor eggs [2017 Abstract] 
Conclusions: This current analysis of IVF outcomes among recipients of vitrified donor oocytes demonstrates that live birth from fresh embryo transfer is not adversely affected by retrieval of more oocytes. Secondarily, there were more surplus embryos for cryopreservation with increasing numbers of retrieved oocytes, increasing the potential cumulative number of infants per retrieval. These findings must be considered in the context of achieving
Can novel early non-invasive biomarkers of embryo quality be identified with time-lapse imaging to predict live birth? 
MAIN RESULTS AND THE ROLE OF CHANCE
Central PN juxtaposition was associated with a 2-fold increase in the odds of live birth (OR ::= ::2.20: 95% CI, [1.26–3.89]: P ::= ::0.006), while the presence of MNB2cell was associated with half the odds of live birth (OR ::= ::0.51: 95% CI, [0.27–0.95]: P ::= ::0.035). These two parameters were independent of embryo kinetics. The 33 remaining parameters had no signif
Male fertility/sperm morphology/oocyte/semen/fertilisation/
Pregnancy following diagnosis of premature ovarian insufficiency: a systematic review 
This review reports pregnancy rates across studies ranging from 2.2% to 14.2%. Mean age in patients who achieved a pregnancy was 30 years, highlighting that oocyte quality in these patients is likely unaffected. No treatment has thus far shown its superiority in improving fertility in women with POI. Recent advances in options such as in-vitro maturation and stem-cell therapy, however, are likely to be the future of treatment and may generate new
The relationship between FSH receptor polymorphism status and IVF cycle outcome: a retrospective observational study 
The homozygous Ser/Ser genotype of FSHR polymorphism at position 680 is associated with a reduced ovarian response to ovarian stimulation in IVF/ICSI. No difference in cumulative live birth rate was found.
Unstable osmolality of microdrops cultured in non-humidified incubators 
Conclusions: Mineral oil alone may not adequately prevent gradual changes in the osmolality of low-volume microdrops during extended in vitro culture of human embryos in non-humidified incubators. As a result, the osmolality may increase to high enough levels to stress some human embryos and adversely affect clinical outcomes. We therefore recommend that the stability of osmolality should be given more consideration to ensure optimal culture cond
Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection 
Conclusion(s). There is a small but increased risk of early miscarriage if HDS >15% compared with HDS ≤::15%. This increased risk is seen only after ICSI, not after IVF. These findings suggest that HDS can be used as a predictor of an increased risk of miscarriage in ICSI treatments
Secretome profile selection of optimal IVF embryos by matrix-assisted laser desorption ionization time-of-flight mass spectrometry 
Conclusion: A simple, direct and rapid analysis of spent culture fluid from blastocysts at the point of embryo transfer can quickly identify optimal embryos with the best chance of achieving ongoing pregnancy. Methods like this, which take less than 20 min to perform, could dramatically improve the approach to embryo selection and live births.
Cell-Free, Embryo-Specific sncRNA as a Molecular Biological Bridge between Patient Fertility and IVF Efficiency 
The aim of this study was to analyze the sncRNA expression profile of the spent culture media on day 4 after fertilization and to reveal a relationship with the morphofunctional characteristics of gametes and resultant embryos, in particular, with the embryo development and implantation potential.
Supraphysiological serum oestradiol negatively affects birthweight in cryopreserved embryo transfers: a retrospective cohort study 
Conclusions: Peak serum oestradiol during COS is negatively associated with birthweight. This model proposes a novel concept as well as new evidence that the effect on birthweight is due to the primary influence of a high oestradiol concentration on oocytes during COS.
Controlled ovarian hyperstimulation (COH) parameters associated with euploidy rates in donor oocytes 
The study provides the first suggestion that patient stimulation parameters can affect the incidence of euploidy in embryos generated through the use of standard assisted reproductive techniques. The study is limited by its retrospective approach and because the aCGH analysis used is less sensitive than more recent NGS technology. Further, it provides a suggestion that the use of hMG is beneficial for obtaining euploid embryos.
Can trophectoderm RNA analysis predict human blastocyst competency? 
In this pilot study, we report that differential gene expression in human trophectoderm biopsies assayed by an ultra-sensitive next generation RNA sequencing strategy could predict blastocyst implantation competence....Following RNA sequencing, a total of 47 transcripts were found to be significantly differentially expressed between the trophectoderm cells from successfully implanted (competent) versus unsuccessful (incompetent) blastocysts.
The present crisis in male reproductive health: an urgent need for a political, social, and research roadmap 
Summary. This paper has presented evidence that there is a present and growing global crisis in male reproductive health. Numerous scientific publications report that chronic illness, disease, and premature death in men are linked to their reproductive health. Further, emerging data demonstrate that the wellness of the female partner, their offspring, and grand‐::offspring all have connections to partner/father reproductive health. These mal
Effect of body mass index on pregnancy outcomes in a freeze-all policy: an analysis of 22,043 first autologous frozen-thawed embryo transfer cycles in 
Conclusions. Among women undergoing first FET with high-quality embryo transfer, low BMI has limited impact on pregnancy and live birth rates. On the contrary, obesity was associated with worse IVF outcomes. Our findings further highlighted that endometrial receptivity played an important role in the poor reproductive outcomes of women with abnormal weight status.