List of Recent Publications. There are 20 links to this search
ID
Publications
Live-cell imaging of human spermatozoa using structured illumination microscopy [2019]
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Here we provide the protocols for and demonstrate live-cell multi-color super-resolution imaging of human spermatozoa using structured illumination microscopy (SIM). By using patches of agarose for immobilization, we achieved four-channel 3D SIM imaging of the plasma membrane, nucleus, mitochondria and microtubulin in the same living sperm cells.
Influence of human embryo cultivation in a classic CO2 incubator with 20% oxygen versus benchtop incubator with 5% oxygen on live births: the randomiz [2019]
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Clinical pregnancy rate showed results in favour of the 5% oxygen group for all subgroups but showed statistical significance only in the day 5 SET subgroup. Culturing of embryos in benchtop incubators under low oxygen produced more blastocysts and therefore was a better alternative for embryo selection, which resulted in higher pregnancy rates. To achieve higher live birth rates, embryo quality is not the only factor.
Can the Sperm Class Analyser (SCA) CASA-Mot system for human sperm motility analysis reduce imprecision and operator subjectivity and improve semen an [2019]
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Compared with the manual WHO method of SA on 4000 semen samples, SCA reduces but does not completely eliminate operator subjectivity. Study SCA and CASA-Mot are useful tools for well-trained staff that allow rapid, high-number sperm motility categorization with less analytical variance than the manual equivalent. Our initial data suggest that SCA motility may have superior predictive potential compared with the WHO manual method for predicating I
Understanding Follicular Output Rate (FORT) and its Implications for POSEIDON Criteria [2019]
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The present review aims to present Follicular Output Rate (FORT) as an efficient quantitative and qualitative marker of ovarian responsiveness to gonadotropins, discuss the underlying mechanisms of impaired sensitivity to FSH and the possible FORT implications for Poseidon criteria.
Is a woman’s chronological age or ‘ovarian age’ more important in determining perinatal outcome after assisted reproductive treatment? [2019]
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Once pregnant, after adjusting for maternal age, women with poor OR were no more likely to experience a biochemical pregnancy or miscarriage than women with normal OR. There were no significant differences in rate) between women with normal or poor OR.
Compromised global embryonic transcriptome associated with advanced maternal age [2019]
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A significant, overall decreased global transcriptome was observed in blastocysts from AMA women. The ESR1/SP1/CREBBP pathway, in particular, was found to be a highly significant upstream regulator impacting biological processes that are vital during embryonic patterning and pre-implantation development. These results provide evidence that AMA embryos are compromised on a cell signaling level which can repress the embryo’s ability to proliferate
Advanced maternal age/ Human blastocyst/ Transcriptome/ Gene expression /
There is another new method for cryopreserving small numbers of human sperm cells [Editorial Commentary] [2019]
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The manuscript by Berkovitz et al. (1), re-opens the debate on a niche, but important, issue in in vitro fertilization concerning the cryopreservation of a very small number of spermatozoa form an ejaculate or with a surgical origin.
Fresh IVF cycles increase risk of SGA, Frozen Cycles increase risk of LGA: Which is Worse? [A comment]. [2019]
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A 2010 retrospective analysis evaluated 123,383 diverse live
births concluding that SGA, but not LGA was associated with increased mortality. In fact, appropriate-for-gestational age (AGA) and LGA neonates had similar likelihoods of death. Therefore, we caution citing aberrant fetal growth as an argument for favoring fresh over
frozen IVF cycles without properly considering other elements that may be more relevant.
Identification of small non-coding RNAs as sperm quality biomarkers for in vitro fertilization [2019]
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In conclusion, we investigated differentially expressed sncRNAs in human sperm as candidate markers for evaluating sperm quality during IVF. We demonstrated that differentially expressed tsRNAs, rsRNAs, and miRNAs are linked to sperm quality according to embryo quality, even though these sperm samples were all considered normal by the traditional semen-parameter assessment. Therefore, the sncRNAs, especially tsRNAs and rsRNAs, may be potential cl
The Discriminatory Legal Barrier of Partner Consent in Victorian ART Law: EHT18 v Melbourne IVF [2019]
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In a single judge ruling, Griffiths J held that the requirement in the Assisted Reproductive Treatment Act 2008 (Vic) (‘ART Act’) for a married woman to obtain the consent of her husband discriminated against the woman in question on the basis of her marital status in contravention of the Commonwealth Sex Discrimination Act 1984 (Cth) (‘SD Act’). His Honour stated the Victorian law in this instance ‘invalid and inoperable’ by operation of section
A familial case report of a 13:22 chromosomal translocation with recurrent intracytoplasmic sperm injection failure [2019]
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We propose the involvement of a balanced t(13:22)(q21.2:q13.3) chromosomal translocation in the pathogenesis of recurrent ART or spontaneous reproductive failures. Hence, it is suggested that all cases with structural chromosomal abnormalities be counseled prior to opting for ART and undergoing pre-implantation genetic diagnosis (PGD).
When my grandchildren in the future ask: ‘Grandad, did you know what you were doing when you worked in the IVF lab?’. The answer is not that easy: I did my best and we helped a lot of children to be conceived, but our databases were less than perfect and we essentially did not know what we exposed gametes and embryos to as the formulation of different culture media was kept secret to us.
The health of children conceived by ART: ‘the chicken or the egg?’ [2019]
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However, studies including children of subfertile couples or of couples treated with milder fertility treatments, such as IUI, as controls show that perinatal risks in these cohorts are lower than for ART children but still higher than for SC indicating that both subfertility and ART influence the future outcome.
Spermatogonial stem cells: updates from specification to clinical relevance [2019]
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This review provides an update including novel views on various aspects of spermatogonial biology (from embryonic to adult stages). We consider this review relevant for all research scientists and clinicians dealing with fertility, spermatogenesis and fertility preservation.
male germline/spermatogonial stem cells/testis/spermatogonia/germ cell niches/heterogeneity/fertility preservation/spermatogenesis/
Sperm donor takes bid to be named girl's father to High Court to stop her mothers moving to New Zealand [2019]
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A man who donated his sperm to a lesbian couple so they could have a child will argue in the High Court today that he is legally the girl's parent and should be able to object to her moving overseas.
Improved in vitro fertilization success and pregnancy outcome with autologous platelet-rich plasma treatment in unexplained infertility patients that [2019]
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We observed that, endometrial thickness was higher after 48 :hours from PRP when compared to endometrial thickness before PRP (10 :mm vs. 6.25 :mm, p :< :.001). Clinical pregnancy rate, and importantly live birth rate were also significantly higher in PRP group than the control group. Based on this information, we showed that intrauterine autologous PRP infusion is a safe, inexpensive adjuvant treatment for optimizing end
The impact of adding hp-hMG in r-FSH started GnRH antagonist cycles on ART outcome [2019]
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In r-FSH :+ :hp-hMG group, significantly higher implantation rates (35.3% vs 24.3%, p=.017), CPRs (51.2% vs 35.8%, p=.015) and lower OHSS rates (1.6% vs 7.4%, p := :.03) were observed respectively compared to r-FSH only treated patients. In conclusion, addition of hp-hMG on the day of antagonist initiation might increase CPRs. A better endometrial receptivity associated with higher implantation rates might be achieved due to h
Time-lapse videography for embryo selection/de-selection: a bright future or fading star? [2019]
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In this commentary issues identified in time-lapse embryo selection/de-selection algorithms, such as quantitative versus qualitative parameters, are discussed alongside the reference start point for the timing system, and types of datasets used for developing and validating time-lapse algorithms. Considering these factors, alternative future research directions which could potentially solve current issues are proposed.
Which embryo selection method should be offered to the patients? [2019]
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We have tools that may change the order in which the embryos are transferred but these technologies will not improve the outcome per treatment started. However, they are likely to lead to live birth sooner and may lower the risk of spontaneous abortion. This comes at the risk of potentially losing a small proportion of embryos along with the possibility of additional financial expenses. We should only offer these add on technologies after proper
Interpregnancy interval and singleton pregnancy outcomes after frozen embryo transfer [2019]
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Conclusion(s): In this nationally representative population, an interval from delivery to start of an FET cycle of <12 months is associated with increased odds for preterm delivery among singleton live births. Consistent with data for patients undergoing fresh IVF, the data support delaying FET 12 months from a live birth.
