Can novel early non-invasive biomarkers of embryo quality be identified with time-lapse imaging to predict live birth?
MAIN RESULTS AND THE ROLE OF CHANCE
Central PN juxtaposition was associated with a 2-fold increase in the odds of live birth (OR ::= ::2.20: 95% CI, [1.26–3.89]: P ::= ::0.006), while the presence of MNB2cell was associated with half the odds of live birth (OR ::= ::0.51: 95% CI, [0.27–0.95]: P ::= ::0.035). These two parameters were independent of embryo kinetics. The 33 remaining parameters had no signif
Male fertility/sperm morphology/oocyte/semen/fertilisation/
Pregnancy following diagnosis of premature ovarian insufficiency: a systematic review
This review reports pregnancy rates across studies ranging from 2.2% to 14.2%. Mean age in patients who achieved a pregnancy was 30 years, highlighting that oocyte quality in these patients is likely unaffected. No treatment has thus far shown its superiority in improving fertility in women with POI. Recent advances in options such as in-vitro maturation and stem-cell therapy, however, are likely to be the future of treatment and may generate new
The relationship between FSH receptor polymorphism status and IVF cycle outcome: a retrospective observational study
The homozygous Ser/Ser genotype of FSHR polymorphism at position 680 is associated with a reduced ovarian response to ovarian stimulation in IVF/ICSI. No difference in cumulative live birth rate was found.
Unstable osmolality of microdrops cultured in non-humidified incubators
Conclusions: Mineral oil alone may not adequately prevent gradual changes in the osmolality of low-volume microdrops during extended in vitro culture of human embryos in non-humidified incubators. As a result, the osmolality may increase to high enough levels to stress some human embryos and adversely affect clinical outcomes. We therefore recommend that the stability of osmolality should be given more consideration to ensure optimal culture cond
Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection
Conclusion(s). There is a small but increased risk of early miscarriage if HDS >15% compared with HDS ≤::15%. This increased risk is seen only after ICSI, not after IVF. These findings suggest that HDS can be used as a predictor of an increased risk of miscarriage in ICSI treatments
Secretome profile selection of optimal IVF embryos by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Conclusion: A simple, direct and rapid analysis of spent culture fluid from blastocysts at the point of embryo transfer can quickly identify optimal embryos with the best chance of achieving ongoing pregnancy. Methods like this, which take less than 20 min to perform, could dramatically improve the approach to embryo selection and live births.
Cell-Free, Embryo-Specific sncRNA as a Molecular Biological Bridge between Patient Fertility and IVF Efficiency
The aim of this study was to analyze the sncRNA expression profile of the spent culture media on day 4 after fertilization and to reveal a relationship with the morphofunctional characteristics of gametes and resultant embryos, in particular, with the embryo development and implantation potential.